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. 2009 Jul 22;83(19):9813–9823. doi: 10.1128/JVI.00550-09

FIG. 1.

FIG. 1.

Diagrams showing genetic organization of rMuVgag (A) and rVSVN4CT1gag1 (B) genomes. Le and Tr are MuV leader and trailer sequences, respectively. An AscI site was generated in the rMuV M gene untranslated region by PCR mutagenesis. The HIV-1 p55 Gag gene with flanking M gene transcription stop (red box) and F gene transcription start (green box) signals was cloned into the AscI site. The rVSVN4CT1gag1 genome had the N gene translocated to position 4 and the HIV-1 p55 Gag gene at position 1 in the genome; the rVSV G gene encoded a truncated form of G protein (hashed box), containing a single amino acid in the cytoplasmic tail.