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One-step growth curves for rMuV and rMuVgag. Replicate Vero cell monolayers in six-well plates were infected in duplicate with rMuV and rMuVgag at an MOI of 5 PFU/cell. Infected cell supernatants were collected at intervals postinfection and titrated by standard plaque assay. Each data point represents the average titer obtained from duplicate infections.
