ICS in immunized animals at week 25 postinoculation. (A) Average percentage of CD8+ T cells expressing IFN-γ, IL-2, and TNF-α, with standard error (error bars). (B) Average percentage of CD4+ T cells expressing IFN-γ, IL-2, and TNF-α, with standard error (error bars).Dots represent individual animals. Proportions of cells expressing individual and multiple cytokines for each vaccination regimen are indicated by the size of colored regions of the pie charts, according to the key at the bottom of each panel.