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. 2009 Jul 15;83(19):9911–9922. doi: 10.1128/JVI.00533-09

FIG. 2.

FIG. 2.

Coreceptor utilization of 239-ST1.2-32. (A) Fusion activity in a cell-cell fusion assay of 239-ST1.2-32. Percent fusion was calculated by using luciferase activity normalized to SIVmac239 fusion with CD4+ CCR5+ QT6 cells or to HIV-1R3A fusion with CD4+ CXCR4+ QT6 cells. Background fusion levels with QT6 cells expressing only CD4 were subtracted prior to normalization. The data shown are means of three experiments plus the standard errors of the means (SEM). (B) Inhibition of 239-ST1.2-32 fusion by AMD3100. Percent fusion was calculated by using luciferase activity normalized to fusion in the absence of inhibitor. The data shown are means of three experiments ± SEM. (C) Inhibition of 239-ST1.2-32 Long CT infection by AMD3100. SupCCR5 and SupT1 cells were infected with SIVmac239 and 239-ST1.2-32 Long CT, respectively, in the presence of the indicated concentrations of AMD3100. RT activity in the culture supernatants was measured at 14 days postinfection. Results from a representative experiment are shown.