FIG. 4.

Singe-round infectivity of NL(AD8)-NX viruses carrying gp41 CTs and matrix proteins derived from HIV-1 present at the early or late stages in patient 153. Virus stocks of the indicated molecular clones, obtained by transfecting 293T (A) and HeLa cells (B) or by infecting MT4.R5 cells (C), PBMC (D), or MDMs (E), were used to infect TZM-bl cells, as described in Materials and Methods. Eight 10-fold serial dilutions of each virus stock were tested in 96-well plates. After 48 h, infection of the target cells was assessed by staining with X-Gal. The infectivity of each virus is expressed as the number of infected cells per ng of p24. The data are expressed as means ± the standard deviations.