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(A) Schematic overview of PrV pUL36 full-length protein (for details see Fig. 1). pUL36Δ290-326 contains a deletion of NLS1 and 2. For immunofluorescence analyses, RK13 cells were transfected with expression plasmids for wild-type pUL36 or pUL36Δ290-326 (B) as well as cotransfected with PrV-ΔUL36F and pcDNA-UL36 (C). PrV-ΔUL36F-transfected cells were detected by indirect immunofluorescence with an anti-gC monoclonal antibody (blue). α-, anti-. Scale bars represent 5 μm.
