FIG. 8.

Immunoelectron microscopy. RK13 cells were infected with PrV-Ka at an MOI of 1 and analyzed 14 h after infection. Ultrathin sections were immunogold labeled with anti-UL36-1 (A and B), anti-UL36-2 (C and D), anti-UL36-3 (E and F), anti-UL36-4 (G and H), and anti-UL31 sera (I and J) (17). Whereas no pUL36-specific label was detected on primary enveloped virions in the perinuclear space with any of the antisera (A, C, E, and G), extracellular wild-type virions were heavily labeled, demonstrating the potency of the antisera (B, D, F, and H). For better visibility, arrows in panels D and H highlight the presence of gold particles. For a control, primary enveloped virions in the perinuclear space but no extracellular virions were labeled with antiserum against pUL31. Bars represent 200 nm.