TABLE 1.
Primers used for amplification of putative NLS
| Oligonucleotide | Sequencea |
|---|---|
| sb_NLS1_FOR | 5′-CACAGAATTCATGCCGCCGCGGGTCCAGAAGC-3′ |
| sb_NLS1_REV1 | 5′-CACAGGATCCTCCTCGAGCTCGTCGGCGTGG-3′ |
| sb_NLS1_REV3 | 5′-CACAGGATCCACCGTGTGCGTGCTGCTCAGGTC-3′ |
| sb_NLS2_FOR | 5′-CACAGAATTCATGCGGCCGTCGCGCAAGTTCC-3′ |
| sb_NLS3_REV | 5′-CACAGGATCCATGTACTCGTGCTGCCCGGCCAG-3′ |
| sb_NLS3_FOR | 5′-CACAGAATTCATGGAGAAGGCGCGCGACACGGG-3′ |
a
Restriction sites for convenient cloning are underlined, and introduced start codons are highlighted in boldface.