TABLE 2.

Pseudovirion neutralization assays of rabbit sera raised against indicated native or denatured BL1-16L2 proteins using Freund's CFA or IFA as the adjuvant^a

Pseudovirions	Neutralizing antibody titer with antiserum to BL1-16L2
	Native 18-31 (protein A)	Denatured 18-31 (protein A)	Native 17-36 (protein B)	Denatured 17-36 (protein B)	Native 75-112 (protein E)	Denatured 75-112 (protein E)	Native 115-154 (protein F)	Denatured 115-154 (protein F)	Native 13-107 (protein I)
HPV16	0	100	1,000	100	100	50	1,000	100	100
	100	100	10,000	100	0	0	100	50	0
HPV18	0	100	100-1,000	100	0	0	0	0	0
	0	100	1,000	0	0	0	0	0	0
HPV31	0	0	0	0	0	x	0	0	0
	0	0	0		x	x	0	x	x
HPV45	0	100	100	0	0	x	0	0	0
	0	0	100-1000		x	x	0	x	x
HPV52	0	0	0	0	0	x	0	0	0
	0	100	100		x	x	0	x	x
HPV58	0	0	100	100	0	x	0	0	50
	0	100	1,000	0	x	x	0	x	x
HPV6	0	0	0	0	0	x	0	0	0
	0	0	0		x	x	0	0	x
HPV11	0	0	0	0	0	x	0	0	0
	0	0	50-100		x	x	0	0	x
HPV5	0	1,000	100	100	0	x	0	0	0
	100	100	10,000	0	x	x	0	x	x
CRPV	0	0	0	0	0	x	0	0	0
	0	50	0		x	x	0	x	x
	Native 2-22 (protein C)	Denatured 2-22 (protein C)	Pentameric 18-31 (protein A)	Native 149-175 (protein G)	Denatured 149-175 (protein G)	Native 172-200 (protein H)	Denatured 172-200 (protein H)
HPV16	0	0	0	0	0	0	0
	0	0	0	0	0	0	0
HPV18	0	0	0	0	0	0	0
	0	0	0	0	0	0	0

^aTiters of two animals each are shown. Sera that neutralized neither HPV16 nor HPV18 were not tested (x) for the remaining pseudovirion types. Assays were performed with serum dilutions ranging from 1:100 to 1:1,000,000. When a lower titer of neutralization was suspected, sera were reevaluated at dilution of 1:50.