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. 2009 Jul 15;83(19):9743–9758. doi: 10.1128/JVI.00125-09

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Copyright © 2009, American Society for Microbiology

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FIG. 1. — Structure of the transgenes. The mCD4/HIV^Nef, CD4E/HIV^Nef, CD4F/HIV^Nef, CD68/HIV^Nef, and CD11c/HIV^Nef DNAs were constructed as described in Materials and Methods. Symbols: En, mouse CD4 enhancer; hCD4 prom, human CD4 promoter; mSi, mouse silencer; mCD4 prom, mouse CD4 promoter; hCD68 prom, human CD68 promoter; CD11c, mouse CD11c promoter; SV40, polyadenylation sequences from SV40; Ex1 and Ex2, Exons 1 and 2. X represents interruption of the ORF of the indicated HIV-1 genes. Restriction sites: A, AatII; Bs, BssHII; Bst, BstXI; RV, EcoRV; Sc, SacI, Sl, SalI; X, XbaI. Thin bars: regulatory sequences from human CD4 (░⃞), mouse CD4 (□), mouse CD11c (▪), or human CD68 (▒) gene. Thick bars: HIV genome; stippled boxes, SV40 sequences; boxes, exons.