TABLE 1.
Thymic cell surface marker analysis in HIVNef Tg micea
| Mouse lineb | Mean no. of cells (10−6) ± SD in different subpopulations
|
CD4/CD8 | Mean fluorescence (%) ± SDc
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Total | CD4+ CD8+ | CD4+ CD8− | CD4− CD8+ | Mean TcR count ± SD | CD4 | CD8 | SpCD4 | ||
| Non-Tg | 120 ± 37 | 99 ± 31 | 10.5 ± 3.4 | 2.3 ± 0.6 | 13.8 ± 5.1 | 4.0 ± 0.8 | 100 | 100 | 100 |
| CD4C/HIVNef | 65 ± 18* | 55 ± 15* | 2.9 ± 1.1* | 2.6 ± 0.6* | 5.4 ± 1.6* | 1.1 ± 0.2* | 37 ± 2.8* | 53 ± 9.4* | 20 ± 4.5* |
| mCD4/HIVNef | |||||||||
| F85838 | 56 ± 26* | 44 ± 21* | 4.6 ± 2.4* | 1.7 ± 0.8* | 5.6 ± 3* | 2.6 ± 0.9 | 46 ± 10* | 52 ± 4* | 25 ± 9* |
| F85840 | 92 ± 49 | 74 ± 42 | 5.4 ± 2* | 3.4 ± 1.1 | 1.6 ± 0.5* | 7.0 ± 3* | 45 ± 7* | 51 ± 7* | 37 ± 13* |
| CD4F/HIVNef | |||||||||
| F78558 | 84 ± 36 | 68 ± 27 | 4.7 ± 2.3* | 4.4 ± 2.0 | 5.6 ± 2.6* | 1.0 ± 0.1* | 36 ± 3.1* | 57 ± 4.5* | 32 ± 10* |
| F95136 | 45 ± 23* | 39 ± 20* | 1.7 ± 0.9* | 1.6 ± 1* | 3.0 ± 1.7* | 1.1 ± 0.1* | 10 ± 0.6* | 64 ± 1.3* | 31 ± 2* |
| CD4E/HIVNef | |||||||||
| F104990 | 30 ± 6.2* | 25 ± 5.0* | 1.2 ± 0.2* | 1.1 ± 0.2* | 2.2 ± 0.4* | 1.1 ± 0.1* | 38 ± 2* | 55 ± 0.7* | 53 ± 13* |
| F104991 | 43 ± 9.6* | 31 ± 7.8* | 4.2 ± 1.4* | 2 ± 0.3 | 4.3 ± 0.9* | 2.2 ± 0.5* | 47 ± 21* | 70 ± 8* | 60 ± 6.1* |
| CD68/HIVNef | |||||||||
| F115706 | 114.9 ± 21.7 | 95.0 ± 17.8 | 9.3 ± 1.2 | 2.7 ± 0.5 | 17.0 ± 2.9 | 4.9 ± 1.6 | 93.7 ± 10.5 | 94.4 ± 9.4 | 100 |
| F115708 | 86.5 ± 9.5 | 70.9 ± 13 | 7.8 ± 1.5 | 3.2 ± 1.3 | 13.6 ± 5.8 | 2.6 ± 0.6 | 78 ± 12 | 82 ± 3 | 100 |
| F115709 | 82.5 ± 30.2 | 68.4 ± 25.5 | 6.7 ± 2.3 | 2.6 ± 1.1 | 12.6 ± 5.3 | 2.6 ± 0.6 | 84.1 ± 11.8 | 99.9 ± 18.4 | 100 |
| CD11c/HIVNef | |||||||||
| F98501 | 88.3 ± 23.0 | 75.3 ± 21.4 | 6.1 ± 0.9 | 1.7 ± 0.4 | 11.2 ± 2.4 | 3.0 ± 0.3 | 99.9 ± 5.9 | 96.7 ± 5.8 | 99.9 ± 18.4 |
| F98506 | 91.4 ± 15 | 73 ± 13 | 12.4 ± 3.1 | 2.0 ± 0.4 | 14.4 ± 3.4 | 5.9 ± 0.9 | 96 ± 4 | 92 ± 2.7 | 100 |
| F98507 | 107 ± 27.1 | 84.6 ± 19.2 | 12 ± 3.8 | 3.5 ± 2 | 14 ± 4.8 | 4.0 ± 1.2 | 100 | 100 | 100 |
*, P < 0.05 according to the Student t test, comparing Tg with non-Tg groups. TcR, T-cell receptor.
FACS analysis was performed on four to nine mice (1.5 to 3 months old) for each Tg line, including the positive control CD4C/HIVNef Tg mice, except for CD11c/HIVNef and CD68/HIVNef mice (both founders), which were analyzed at 2 to 4 months of age. The non-Tg control values were obtained by pooling the results of all non-Tg littermates from different lines.
The mean fluorescence values for CD4 and CD8 in DP CD4+ CD8+ and CD4 in SP T cells were obtained by calculating the ratio of CD4 or CD8 staining in Tg thymuses relative to those of non-Tg thymuses (100%). Mean values were then calculated with the values for each line.