TABLE 2.

Peripheral LN cell surface marker analysis in HIV^Nef Tg mice^a

Mouse lineb	Mean no. of cells (10−6) ± SD in different subpopulations				Mean CD4/CD8 ratio ± SD	Mean fluorescence (%) ± SDc
	Total	CD4+	CD8+	TcR		CD4	CD8
Non-Tg	17.4 ± 5.2	9.0 ± 2.5	3.8 ± 1.9	13.0 ± 4.8*	2.5 ± 0.5*	100	100
CD4C/HIVNef	5.8 ± 3.9*	0.9 ± 0.6*	1.4 ± 1*	2.1 ± 1.5*	0.6 ± 0.1*	15 ± 9*	80 ± 20
mCD4/HIVNef
F85838	3.9 ± 1.8*	0.6 ± 0.3*	1.0 ± 0.5*	1.6 ± 0.8*	0.5 ± 0.1*	30 ± 5*	95 ± 5
F85840	3.3 ± 0.7*	0.3 ± 0.1*	0.9 ± 0.4*	1.4 ± 0.5*	0.5 ± 0.2*	21 ± 13*	92 ± 2
CD4F/HIVNef
F78558	3.8 ± 1.4*	1.1 ± 0.4*	1.2 ± 0.4*	2.3 ± 0.9*	0.9 ± 0.1*	16 ± 3*	90 ± 10
F95136	6.0 ± 3.5*	0.8 ± 0.7*	1.7 ± 0.1*	2.7 ± 1.8*	0.4 ± 0.2*	21 ± 9*	97 ± 7
CD4E/HIVNef
F104990	3.6 ± 0.9*	0.7 ± 0.2*	0.9 ± 0.2*	1.6 ± 0.4*	0.8 ± 0.1*	26 ± 8*	75 ± 11*
F104991	6.8 ± 0.3*	1.1 ± 0.4*	1.2 ± 0.1*	2.1 ± 0.3*	0.9 ± 0.4*	28 ± 20*	70 ± 2*
CD68/HIVNef
F115708	11.1 ± 1.1*	5.7 ± 0.3*	2.2 ± 0.2	8 ± 0.5*	2.5 ± 0.2	93	91
F115709	12.8 ± 5	6.1 ± 2.1	3.1 ± 1.4	6.9 ± 5	2 ± 0.1	100	90
CD11c
F98506	15 ± 6.2	8.4 ± 4.3	3.2 ± 1.4	11.6 ± 5.6	2.5 ± 0.4	100	100
F98507	10.8 ± 1.7*	5.9 ± 1.0*	2.1 ± 0.6	8.1 ± 1.6*	2.8 ± 0.3	100	100

^a*, P < 0.05 according to the Student's t test, comparing Tg with non-Tg growth.

^bFACS analysis was performed on four to nine mice (1.5 to 3 months old) for each Tg line, except for CD4F/HIV^Nef (F95136) and CD68/HIV^Nef mice (both founders), which were analyzed at 2 to 4 months of age. The non-Tg control values were obtained by pooling the results of all non-Tg littermates from different lines.

^cThe mean fluorescence values for CD4 and CD8 T cells were obtained by calculating the ratio of Tg CD4 or CD8 relative to that of non-Tg lymph nodes (100%). Mean values were then calculated with the values for each line.