TABLE 4.
Viable CFU in organs of HIVNef Tg mice orally infected with C. albicans
| Variable | Mouse typea
|
||||||
|---|---|---|---|---|---|---|---|
| CD4C/HIVNef | CD4E/HIVNef | CD4F/HIVNef | mCD4/HIVNef | hCD68/HIVNef | CD11c/HIVNef | Control non-Tg | |
| No. of animals inoculated | 6 | 6 | 10 | 7 | 8 | 10 | 42 |
| Age at inoculation (days) | 76 | 78 | 36-76 | 130-133 | 66-89 | 76-82 | 36-130 |
| Mean age at assessment in days (range) | 293 (194-357)b | 189c | 175 (149-189)d | 275 (273-276)e | 189 (178-201)f | 193 (192-198)g | 195 (148-293)h |
| Organs culture positive for C. albicans | |||||||
| Brain | |||||||
| No. of mice | 2 | 1 | 0 | 0 | 2 | 0 | 0 |
| Mean C. albicans count (CFU/g) | 1.8 × 103 | 6.9 × 103 | NA | NA | 1.8 × 102 | NA | NA |
| Range (CFU/g) | 1.5 × 103-2.1 × 103 | NA | NA | NA | 3.1 × 101-3.2 × 102 | NA | NA |
| Lungs | |||||||
| No. of mice | 1 | 1 | 1 | 0 | 2 | 0 | 0 |
| Mean C. albicans count (CFU/g) | 4.5 × 103 | 1.8 × 103 | 8.3 × 100 | NA | 1.1 × 102 | NA | NA |
| Range (CFU/g) | NA | NA | NA | NA | 7.0 × 101-1.5 × 102 | NA | NA |
| Liver | |||||||
| No. of mice | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| Mean C. albicans count (CFU/g) | 4.5 × 102 | NA | NA | NA | NA | NA | NA |
| Range (CFU/g) | 2.7 × 102-6.3 × 102 | NA | NA | NA | NA | NA | NA |
| Kidneys | |||||||
| No. of mice | 2 | 1 | 0 | 0 | 1 | 0 | 0 |
| Mean C. albicans count (CFU/g) | 2.8 × 102 | 6.9 × 102 | NA | NA | 5.0 × 101 | NA | NA |
| Range (CFU/g) | 1.1 × 102-4.4 × 102 | NA | NA | NA | NA | NA | NA |
| Stomachi | |||||||
| No. of mice | 5 | 4 | 5 | 7 | 7 | 6 | 9 |
| Mean C. albicans count (CFU/g) | 1.0 × 105 | 2.3 × 105 | 1.7 × 105 | 2.2 × 105 | 7.2 × 104 | 1.1 × 105 | 1.6 × 105 |
| Range (CFU/g) | 7.9 × 102-4.0 × 105 | 4.4 × 104-5.3 × 105 | 4.1 × 104-4.7 × 105 | 2.5 × 104-4.9 × 105 | 9.1 × 101-5.8 × 105 | 2.0 × 102-3.4 × 105 | 1.3 × 104-3.8 × 105 |
| Small intestinei | |||||||
| No. of mice | 5 | 6 | 5 | 7 | 6 | 7 | 9 |
| Mean C. albicans count (CFU/g) | 2.0 × 103 | 2.0 × 104 | 2.6 × 103 | 3.3 × 103 | 5.0 × 103 | 8.1 × 102 | 1.6 × 103 |
| Range (CFU/g) | 5.9 × 101-6.5 × 103 | 1.2 × 104-3.8 × 104 | 2.9 × 102-7.9 × 103 | 6.5 × 101-1.1 × 104 | 3.2 × 101-8.1 × 103 | 3.3 × 101-1.7 × 103 | 6.6 × 101-9.2 × 103 |
| Large intestinei | |||||||
| No. of mice | 5 | 4 | 5 | 7 | 6 | 8 | 9 |
| Mean C. albicans count (CFU/g) | 3.4 × 104 | 8.6 × 104 | 1.0 × 104 | 8.3 × 103 | 2.3 × 104 | 9.4 × 103 | 6.1 × 103 |
| Range (CFU/g) | 8.5 × 102-1.2 × 105 | 2.8 × 104-1.9 × 105 | 3.2 × 103-2.4 × 104 | 1.2 × 103-2.3 × 104 | 5.3 × 102-9.6 × 104 | 4.0 × 101-4.3 × 104 | 7.1 × 102-1.3 × 104 |
Mice studied included Tg and control non-Tg offspring derived from founder mouse F27367 (CD4C/HIVNef), F104990 (CD4E/HIVNef), F95136 (CD4F/HIVNef), F85840 (mCD4/HIVNef, high expresser) and F69457 (mCD4/HIVNef, low expresser), F115709 (hCD68/HIVNef), and F98507 (CD11c/HIVNef). NA, not applicable.
Four mice were nonsurvivors (assessments, days 194 to 338); two surviving mice were euthanized (assessment, day 357).
All six mice survived and were euthanized at 189 days of age.
All 10 mice survived and were euthanized at 149 to 189 days of age.
All seven mice survived and were euthanized at 273 to 276 days of age.
All eight mice survived and were euthanized at 178 to 201 days of age.
All 10 mice survived and where euthanized at 192 to 198 days of age.
Control non-Tg littermates were euthanized on the same day as the Tg mice.
Note that clearance of C. albicans from stomach and intestine of some non-Tg mice occurs more slowly than from the oral cavity, but without causing mucosal candidiasis as in Tg mice.