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Strategy for construction of the HCMV recombinant FLAG-US28/1-314 in HCMV FIX-BAC. (A) Schematic of US28 depicting the Ser/Thr-rich carboxy-terminal regulatory region. Amino acids 315 to 354 that are deleted in the US28/1-314 mutant are expanded underneath the diagram. (B) Schematic representation of the recombination strategy used to manipulate US28 and construct the US28/1-314 mutant.
