FIG. 4.

Endocytic properties of US28/WT and US28/1-314 proteins in HCMV-infected cells. HFFs were infected with the HCMV FLAG-US28/WT or HCMV FLAG-US28/1-314 viruses for 48 h. Infected cells were then labeled at 4°C with [¹²⁵I]CCL5, washed, and then warmed to 37°C for various time to allow for endocytosis of the US28 proteins. Internalized ligand was assessed by acid washing to remove surface-bound ligand and is presented as the percentage of total ligand bound. The data shown are derived from six independent experiments performed in duplicate and represent the means ± the SEM.