FIG. 5.

US28/1-314 exhibits increased levels of constitutive PLC-β signaling in HCMV-infected cells. HFFs were infected with increasing MOIs (0.03 to 0.3 PFU/cell) using HCMV FLAG-US28/WT or HCMV FLAG-US28/1-314 viruses. Medium containing 1 μCi of [³H]myoinositol/ml was added at 24 h postinfection, and the accumulated inositol phosphates were determined at 48 h postinfection by using anion-exchange chromatography. The data shown are derived from 10 independent experiments performed in duplicate and represent the means ± the SEM.