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. 2009 Jul 15;83(19):10016–10027. doi: 10.1128/JVI.00354-09

FIG. 8.

FIG. 8.

CCL5 and CX3CL1 binding to US28 triggers calcium release by activating PTx-insensitive Gq proteins. (A) HFFs were infected with HCMV FLAG-US28/WT or HCMV FLAG-US28/1-314 viruses at an MOI of 3 and left either untreated (solid symbols) or treated overnight with 200 ng of PTx/ml (open symbols). At 48 h postinfection, cells were labeled with Fluo-4 AM and stimulated with 10 nM CCL5 (top panel) or 10 nM CX3CL1 (lower panel), and the calcium flux was measured by using a FlexStation II fluorometer. (B) To control for the effects of PTx, uninfected HFFs left untreated (closed symbols) or treated with 200 ng of PTx/ml (open symbols) were stimulated with 10 nM LPA, and the calcium flux was measured as described above. The calcium traces are representative of at least four independent experiments performed in duplicate.