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. 2009 Jul 15;83(19):10016–10027. doi: 10.1128/JVI.00354-09

FIG. 9.

FIG. 9.

CCL5 and CX3CL1 binding to US28 triggers calcium release via a PLC-β/IP3 signaling pathway. HFFs were infected with HCMV FLAG-US28/WT or HCMV FLAG-US28/1-314 viruses at an MOI of 3. (A) At 48 h postinfection, cells were labeled with Fluo-4 AM in the absence (closed symbols) or presence (open symbols) of the PLC-β inhibitor U73122. Cells were then stimulated with 10 nM CCL5 (upper panel) or 10 nM CX3CL1 (lower panel) and analyzed by using a FlexStation II fluorometer. (B) At 48 h postinfection, cells were labeled with Fluo-4 AM in the absence (closed symbols) or presence (open symbols) of the IP3 channel blocker 2-ABP. Cells were then stimulated with 10 nM CCL5 (upper panel) or 10 nM CX3CL1 (lower panel) and analyzed by using a FlexStation II fluorometer. The calcium traces shown are representative of at least four independent experiments performed in duplicate.