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Schematic representation of two different strategies of membrane anchoring of MPER peptides. (A) Nonphysiologic anchoring at the amino terminus of MPER used in our earlier report (2). (B) Physiologic anchoring at the carboxyl terminus of MPER used in this study. The hydrophobic membrane-anchoring sequence is shown as a rectangle and was attached at either the amino or carboxyl terminus of the MPER peptide, which is shown as a black line.
