FIG. 3.

Temperature dependence of 2F5 and 4E10 binding to peptide-liposome conjugates. Binding kinetics of 2F5 (A and B) and 4E10 (C and D) were measured at different temperatures, as indicated, for the biepitope (A and C) and 2F5 and 4E10 nominal epitope (B and D) peptide-lipid conjugates. The specific binding signal was recorded with reference to the signal for peptide-free synthetic liposomes, as described in Materials and Methods. The data recorded at different temperatures were normalized and are presented as the percent response. RU, relative units.