FIG. 5.

Evaluation of exposure of membrane-anchored 2F5 and 4E10 nominal epitope and biepitope peptides to aqueous quencher. (A to C) Tryptophan fluorescence emission spectra of 2F5 nominal epitope (A), 4E10 nominal epitope (B), and biepitope (C) peptide-liposome conjugates at different added acrylamide concentrations. a.u., arbitrary unit. (D to F) Stern-Volmer plots for the quenching experiments whose results are shown in panels A to C. Ratio of tryptophan fluorescence intensity of peptide-liposomes in the absence of acrylamide to that at different acrylamide concentrations (F₀/F) as a function of acrylamide concentration.