FIG. 3.

Timing, dose, and STAT1 dependence of antialphavirus ISG induction. (A and B) Neuron cultures were either untreated (M) or treated with the indicated doses of IFN-α/β for the times indicated. Total cellular RNA was then harvested and subjected to semiquantitative RT-PCR for each ISG and the β-actin control as described in Materials and Methods. (C) Neurons from wild-type (STAT1^+/+) or STAT1^−/− mice were mock treated or treated with 1,000 IU/ml of IFN-α/β for 6 h, and then RNA was harvested and semiquantitative RT-PCR was performed as described for panels A and B.