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Effects of VEEV or SINV infection upon IFN-β gene and ISG transcription in IFN-pretreated neurons. Neuron cultures were either untreated or treated with 1,000 IU/ml of IFN-α/β for 24 h and then either mock infected (M) or infected with VEEV (left panel) or SINV (right panel) as described in Materials and Methods. Total cellular RNA was harvested at the times indicated and processed for semiquantitative RT-PCR for the indicated mRNAs as described in Materials and Methods.
