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MNV-1 nonstructural proteins all exhibit a similar subcellular localization pattern in infected cells. RAW264.7 cells were infected with MNV-1 at an MOI of 5; fixed at 12 hpi; and dual labeled with antibodies specific for dsRNA and for MNV-1 nonstructural proteins NS1-2, NS3, NS4, NS5, and NS6. Antibodies were subsequently visualized with species-specific IgG conjugated to Alexa Fluor 488 or 594.
