FIG. 4.

The MNV-1 RC associates with membrane vesicles in infected cells. Cryosections of RAW264.7 cells infected with MNV-1 at an MOI of 5 and fixed at 12 hpi (A, C, E, and F) or 24 hpi (B and D) were immunolabeled with anti-dsRNA antibodies and 10-nm PAG (A and B) or anti-NS7 antibodies and 10-nm PAG (C and D). In panels E and F, the cryosections from MNV-1-infected RAW264.7 cells were dual labeled with antibodies to dsRNA (5-nm PAG) and NS7 (15-nm PAG). Arrows in panels E and F highlight the smaller 5-nm gold particles. Magnification bars represent 200 nm in all cases.