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. 2009 Jul 8;83(19):9709–9719. doi: 10.1128/JVI.00600-09

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Copyright © 2009, American Society for Microbiology

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FIG. 5. — MNV-1 associates with markers for the ER, cis/medial-Golgi bodies, and endosomes in infection. RAW264.7 cells were infected with MNV-1 at an MOI of 5, fixed at 12 hpi, and dual labeled with markers for the MNV-1 RC (dsRNA or NS7, shown in green) and various cellular membrane markers (shown in red). The MNV-1 RC was found to partially colocalize with markers for the ER (calnexin), the cis/medial-Golgi apparatus (Giantin) and endosomes (EEA1), visualized as a yellow hue, but showed very little colocalization with the cis Golgi (GM130) and no colocalization with lysosomes (LAMP1).