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. 2009 Jul 15;83(19):9923–9939. doi: 10.1128/JVI.00066-09

FIG. 4.

FIG. 4.

Characterization of the 191 core synthesized in the presence of (Z-LL)2-ketone. (A) 293T cells expressing the 191 core were treated with or without 100 μM (Z-LL)2-ketone at 37°C for 14 h, and the postnuclear extracts were analyzed by Optiprep-based subcellular fractionation. Migration of the innate p23 and processed p21 core proteins is marked. Percentages of core proteins distributed in soluble fractions 16 to 22 of the total core populations are indicated on the right of each panel. (B) 293T cells expressing the 191 core were treated with or without 20 μM (Z-LL)2-ketone at 37°C for 20 h, and the postnuclear extracts were subjected to a proteinase K digestion assay. The amount of the core synthesized in the presence of the SPP inhibitor was adjusted for better comparison of the protease resistance to that of the core expressed without the SPP inhibitor. (C) Cells expressing the 191 core were treated with 20 μM (Z-LL)2-ketone for 20 h or untreated, and cell lysates were subjected to 5 to 50% sucrose gradient centrifugation.