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. 2009 Jul 22;83(19):10187–10197. doi: 10.1128/JVI.00396-09

FIG. 7.

FIG. 7.

pUL29 and pUL29/28 are present in HCMV virions. (A) Partially purified BADinUL29F virion preparations contain pUL29/28F. Virions were solubilized, and serial dilutions, as well as an infected cell lysate control, were analyzed by Western blotting with antibodies to the FLAG epitope and the known virion proteins pUL69, pUL44, pUL83, and pUL99. pUL123 was assayed as a negative control. (B) pUL29F and pUL29/28F are located within the virion envelope. inUL29F virions were treated with increasing amounts of trypsin (T), with trypsin and Triton X-100 (TT), or untreated (U). Virions were then solubilized, and proteins were analyzed by Western blotting with a monoclonal antibody specific to the FLAG epitope and, as a control, a monoclonal antibody to pUL99.