FIG. 1.

ICP0 expression in HaCaT cells and primary human and mouse keratinocytes. Subconfluent HaCaT cells (A and B), primary human keratinocytes (D), or primary mouse keratinocytes (E) were infected with HSV-1 (20 PFU/cell) and fixed at 1, 2, 3, and 4 h p.i., respectively. F-actin was visualized with TRITC-phalloidin (red). Cells were stained with mouse anti-ICP0 (green), visualized with Alexa Fluor 488-conjugated anti-mouse immunoglobulin G (Molecular Probes). Total protein extracts were prepared from uninfected HaCaT cells (mock) and from cells at 1, 2, 3, and 4 h p.i. Proteins were resolved on SDS-PAGE gels (10%) and stained with rabbit anti-ICP0 antiserum. Staining with rabbit anti-PDI (protein disulfide isomerase) antiserum was used as a loading control. Single stainings and merged images of confocal projections are shown. Bar, 50 μm (A and B) or 80 μm (D and E).