Table 1.
Primary antibody and antibody/fluorochrome-conjugates against various immune mediators, B. burgdorferi, and cell phenotypes
| MEDIATOR/CELL TYPE | PRIMARY AB/SOURCE | ISOTYPE | DILUTION/CONCENTR. |
| IL-6 | anti-human IL-6 (Abcam Inc. Cambridge, MA) |
Rabbit IgG | 1:1000 |
| IL-8 | anti-human IL-8 RDI Flanders, NJ |
Rabbit IgG | 10 μg/ml |
| CCL2 | anti-human CCL2 (BD) |
Mouse IgG1 | 10 μg/ml |
| anti-human CCL2 (Abcam) |
Rabbit IgG | 1:10 | |
| CXCL13 | anti-human CXCL13 (R&D) |
goat IgG | 5 μg/ml |
| B. burgdorferi | anti-whole cell preparation (Accurate Chemicals, Westbury, NY) |
Rabbit IgG | 1:250 |
| anti- B. burgdorferi-FITC (Kirkegaard and Perry, Gaithersburg, MD) |
Goat | 1:10 | |
| Astrocyte | anti-human GFAP-cy3 (Sigma St Louis, MO) |
Mouse IgG1 | 1:200 |
| anti-human GFAP (Sigma) |
Mouse IgG1 | 1:200 | |
| Astrocyte, Oligodendrocyte, Schwann cell, satellite cell | anti-bovine S-100 (Sigma) |
Rabbit IgG | 1:1000 |
| Microglia | anti-Iba1 synthetic peptide (Wako Pure Chemicals, Richmond, VA) |
Rabbit IgG | 1:100 |
| Endothelial cells | anti-human GLUT-1 (Chemicon) |
Rabbit IgG | 1:50 |
| Neuron | anti-mouse NeuN (Chemicon) |
Mouse IgG1 | 1:20 |
| T cell | anti-human CD3 (Dako, Carpinteria, CA) |
Mouse IgG2a | 1:10 |
| B cell | anti-human CD20 (Dako) |
Mouse IgG1 | 1:200 |
| Macrophage | anti-human CD68 (Dako) |
Mouse IgG1 | 1:50 |