Table 2.

Correlations between SP properties and measured extracellular NucA activities.^a

Ranked after NucA activityb	Lenght of SPs	pI of SPs	Hydro-phobicityc	Length of N-domaind	Net charge of the N-domaind	Charge/length of the N-domaind	Length of H-plus C-domainsd
1-10	34.5	9.8	2.98	7.2	3.1	0.5	27
65-78	32.8	10.1	2.31	9.9	3.0	0.4	23
1-39	31.6	10.3	2.77	7.4	2.9	0.5	24
40-78	31.1	10.1	2.26	9.1	3.0	0.4	22
1-78	31.3	10.2	2.52	8.3	3.0	0.4	23

^aAverage values for different properties were calculated for SPs grouped by NucA activity.

^bSubgroups of the 78 SPs were made as indicated, with SPs being numbered by their ability to produce extracellular NucA activity, with SP number 1 having the highest activity; see text for further details.

^c The hydrophobicity of the SPs was estimated using the ProtScale program [66] using the Kyte & Doolittle scale [67] on the ExPASy Server http://www.expasy.ch/tools/protscale.html. The numbers indicate the maximum hydrophobicity value, using a sliding window size of seven.

^d See text for definition of the domains. Nb. We verified that the length of the H+C domain is not correlated to the maximum hydrophobicity defined in footnote c (results not shown).