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. Author manuscript; available in PMC: 2009 Sep 21.
Published in final edited form as: J Med Chem. 2008 Aug 5;51(16):5052–5063. doi: 10.1021/jm8003366

Figure 2. RFC transport and FR binding activities for cell line models.

Figure 2

Assorted cell line models including R2, PC43-10, RT16, D4, KB, and IGROV1 were assayed for [3H]MTX uptake as a measure of RFC levels and [3H]folic acid binding as a measure of FR levels. Assays were performed as described in the Experimental Section. For both transport and binding assays, radioactive substrate was expressed in units of pmol/mg protein. Abbreviation: FA, folic acid. Results are presented as mean values plus/minus SEM from 3 experiments.