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. Author manuscript; available in PMC: 2009 Sep 21.
Published in final edited form as: J Struct Biol. 2007 Jun 28;159(3):507–522. doi: 10.1016/j.jsb.2007.06.006

Fig. 8.

Fig. 8

(a) Simulated STEM image of an Undecagold cluster in a 40 nm-thick carbon film. This image was obtained with a single dose of 105 e/nm2 and an inner collection semi-angle of 16 mrad. (b) Simulated zero-tilt projection image obtained with a fractionated dose of 2.1×103 e/nm2; (c–e) one nanometer thick slices from the STEM tomogram reconstructed with SIRT; (f–h) one nanometer thick slices from the STEM tomogram reconstructed with WBP in IMOD. The gold particles shown in (f), (g), and (h) were extracted from the same locations as those shown in (c), (d), and (e). The 2D SNR of the gold particle in (a) is 8.9, which is comparable with the 3D SNR of 6.4 ± 0.3 and 5.1 ± 0.3 for the SIRT and WBP reconstructions, respectively. As the images and SNR values suggest, gold particles in 3D are better visualized using SIRT for the reconstruction.