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. Author manuscript; available in PMC: 2010 Oct 9.
Published in final edited form as: J Mol Biol. 2009 Jul 1;392(5):1178–1191. doi: 10.1016/j.jmb.2009.06.064

Figure 1.

Figure 1

13C CPMAS echo spectra of E. faecium whole cells enriched with D-[1-13C]alanine, grown in the presence of alaphosphin and no vancomycin (black) or 25 μg/ml vancomycin (red). The spectra are normalized with respect to the natural-abundance, aliphatic-carbon signal intensities between 0 and 35 ppm. The line shape of the peak centered around 175 ppm changes with the addition of vancomycin (inset). The D-Ala contribution at 178 ppm, whose signal results from a terminal carboxyl D-Ala, increases with vancomycin exposure as transglycosylase is inhibited and Park's nucleotide accumulates in the cytoplasm.