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. 2009 Jun 24;29(25):8236–8247. doi: 10.1523/JNEUROSCI.1805-09.2009

Figure 2.

Figure 2.

Inhibition of PI3′K reduces FOXO3a phosphorylation but does not protect against mutant SOD toxicity. Mixed spinal cord cultures were grown in the absence of BDNF and pretreated with LY294002 or vehicle before acute stimulation with BDNF. Thirty minutes after application of BDNF, lysates were prepared for Western blot analysis. A shows that LY294002 blocks the BDNF stimulated increase in phospho-AKT, phospho-MAPK, and phosphorylation of FOXO3a on T32 and S253. B shows the effect of a 24 h treatment with LY294002 of cultures chronically grown in five trophic factors, including BDNF. By Western blot analysis, LY294002 suppresses phospho-AKT and phosphorylation of FOXO3a on S253. C shows the effect of a 6 d treatment with LY294002 of cultures chronically grown in five trophic factors, including BDNF. By Western blot analysis, LY294002 suppresses phospho-AKT, phospho-MAPK, and phosphorylation of FOXO3a on T32 and S253. D shows the number of motor neurons surviving 6 d of infection with HSV–WT-SOD or HSV-mutant SOD grown in the presence or absence of chronic LY294002. Mutant SOD led to ∼50% motor neuron loss, and LY294002 did not abrogate the process.