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. 2009 Sep 17;28(21):3315–3328. doi: 10.1038/emboj.2009.267

Figure 1.

Figure 1

β2AR single-cysteine constructs and FRET donor–acceptor pair. (A) Three single-reactive cysteines constructs were generated on a minimal cysteine background (Δ5-β2AR). The labelling sites were placed in the first ICL, Δ5-β2AR-T66C, at the cytoplasmic end of the sixth transmembrane segment, Δ5-β2AR-A265C, and helix eight, Δ5-β2AR-R333C. (B) Intracellular 3D view of the distribution of regions chosen for single-cysteine mutants, α-carbons are depicted. (C) FRET donor (λex=549 nm; λem=570 nm) and acceptor pair (λex=650 nm; λem=670 nm).