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. Author manuscript; available in PMC: 2010 Jul 14.
Published in final edited form as: Biochemistry. 2009 Jul 14;48(27):6469–6481. doi: 10.1021/bi900661b

Figure 3.

Figure 3

HPLC chromatogram of the N-acetyl-d-Xaa library treated with no enzyme (black dots) and 20 nM Bb3285 (red line) for 1 hour at 30 °C. The OPA-derivatized d-glutamate was detected at a retention time of 1.7 minutes in the sample treated with Bb3285. The internal standard is labeled as IS. Additional details are provided in the text.