Table 4.
Kinetic parameters for Gox1177 with selected substratesa
Compound | kcat, s−1 | Km, mM | kcat/Km, M−1s−1 |
---|---|---|---|
N-acetyl-d-Ala | 50 ± 4 | 18 ± 2 | (2.9 ± 0.4) × 103 |
N-acetyl-d-Val | 16 ± 1 | 4.1 ± 0.2 | (3.9 ± 0.2) × 103 |
N-acetyl-d-Leu | 101 ± 3 | 3.2 ± 0.2 | (3.2 ± 0.2) × 104 |
N-acetyl-d-Met | 40 ± 2 | 1.8 ± 0.3 | (2.2 ± 0.4) × 104 |
N-acetyl-d-His | nd | nd | (8.5 ± 0.2) × 103 |
N-acetyl-d-Trp | 89 ± 2 | 2.2 ± 0.2 | (4.1 ± 0.3) × 104 |
N-acetyl-d-Phe | 59 ± 2 | 2.5 ± 0.3 | (2.4 ± 0.3) × 104 |
N-acetyl-d-Tyr | 66 ± 2 | 7.1 ± 0.5 | (9.3 ± 0.7) × 103 |
N-acetyl-d-Thr | nd | nd | (6.0 ± 0.1) × 103 |
N-acetyl-d-Asn | nd | nd | 480 ± 20 |
N-acetyl-d-Gln | nd | nd | (2.7 ± 0.1) × 103 |
N-formyl-d-Leu | 13 ± 1 | 5.9 ± 0.5 | (2.2 ± 0.2) × 103 |
N-succinyl-d-Leu | nd | nd | 30 ± 1.4 |
N-propionyl-d-Leu | 23 ± 1 | 1.2 ± 0.1 | (2.0 ± 0.1) × 104 |
l-Leu-d-Leu | 31 ± 1 | 3.3 ± 0.2 | (9.2 ± 0.7) × 103 |
l-Met-d-Leu | 20 ± 1 | 1.6 ± 0.2 | (1.3 ± 0.2) × 104 |
l-Tyr-d-Leu | 19 ± 1 | 1.3 ± 0.2 | (1.5 ± 0.2) × 104 |
From fits of the data to equation 2.