Skip to main content
. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Cancer Res. 2009 Aug 25;69(17):7111–7120. doi: 10.1158/0008-5472.CAN-08-3957

Figure 5.

Figure 5

Functional TGF-β overexpression by contained prostate epithelial glands. (a) HA-tagged, constitutively active TGF-β1 was constitutively expressed in BPH1 human prostate epithelia. (b) Conditioned media from BPH1-TGFb1 (right), but not BPH1 (left) cells in culture activated a RFP TGFbeta reporter in serum-starved human prostate fibroblasts. (c) Kidney capsule tissue recombination grafting shows a decrease in graft size when rUGM is recombined with BPH1-TGFb1 (right) versus parental control (left). Trichrome (d) and smooth muscle alpha actin (e) staining reveals predominantly smooth muscle surrounding BPH1 acini (left) verses increased periacinar collagen deposition in BPH1-TGFbeta grafts (right).