Table 1.
Primer sets of real-time PCR
| Genes | Forward (5′ → 3′) | Reverse (5′ → 3′) |
|---|---|---|
| LXRα | GCCGAGTTTGCCTTGCTCA | TCCGGAGGCTCACCAGTTTC |
| SREBP-1c | GGCTCCTGCCTACAGCTTCT | CAGCCAGTGGATCACCACA |
| SREBP-2 | ACAACCCATAATATCATTGAGAAACG | TTGTGCATCTTGGCGTCTGT |
| MDR3 | GCCGAGTTTGCCTTGCTCA | TCCGGAGGCTCACCAGTTTC |
| LDLR | CAACGGCTCAGACGAGCAAG | AGTCACAGACGAACTGCCGAGA |
| BSEP | CTTCATCATGGACCTGCCACA | GGATGAGGGCTCTGGCGATA |
| FXR | ACCTCGACAACAAAGTCATGCAG | ATTGGTTGCCATTTCCGTCA |
| CYP7A1 | AGCATTTGTGAATACATGGCTGGA | TTCACAAGCAAGCACTGGTGAAC |
| ABCG5 | ATTGTGGTTCTCACCATTCACCAG | GGTTTGAATGTTCAGGACAAGGGTA |
| ApoB | TCAAGAGTTACAGCAGATCCATCAA | TCAGAATGGAAGTCCTTAAGAGCAA |
| MTP | AGCACCTCAGGACTGCGAAGA | CAGAGGTGACAGCATCCACCA |
| HMGR | GCCTGGCTCGAAACATCTGAA | CTGACCTGGACTGGAAACGGATA |
| NPC1L1 | TCTGTGGAGTTTGTGTCCCACATTA | GTTGGTCATGGCCACACCTG |
| PPARα | CAGCCTGTGGCCTCTGTAGTT | CCCTTTACACAACCGAAGTTCCT |
| β-actin | TGGCACCCAGCACAATGAA | CTAAGTCATAGTCCGCCTAGAAGCA |
LXR liver X receptor, SREBP sterol regulatory element-binding protein, MDR multidrug resistance, BSEP bile salt export pump, FXR farnesoid X receptor, CYP7A1 cholesterol 7α-hydroxylase, ABCG5 ATP-binding cassette G5, ApoB apolipoprotein B, MTP microsomal triglyceride transfer protein, HMGR HMG-CoA reductase, NPC1L1 Niemann-Pick C1 like 1, PPAR peroxisome proliferator-activated receptor