Table 2.
Associations between IL1RN tagSNP genotype and plasma log(CRP) levels in CARDIA and in CLEAR and CHS validation populations
| IL1RN tag SNP (rs id) | Genotype | CARDIA β ± SE (p-value) |
CLEAR β ± SE (p-value) |
CHS β ± SE (p-value) |
Pooled estimate β [95% CI] (p-value) |
|---|---|---|---|---|---|
| 1018 (rs4251961) | T/T | 0 | 0 | 0 | |
| T/C | −0.034 ± 0.056 (0.55) | 0.011 ± 0.056 (0.89) | 0.092 ± 0.032 (0.004) | ||
| C/C | 0.195 ± 0.084 (0.02) | 0.201 ± 0.084 (0.06) | 0.109 ± 0.045 (0.017) | ||
| per C allele | 0.062 ± 0.039 (0.11) | 0.082 ± 0.051 (0.11) | 0.067 ± 0.021 (0.002) | 0.068 [0.034 – 0.102] | |
| P<0.0001 | |||||
| 2765 (rs315919) | G/G | 0 | 0 | ||
| G/T | −0.091 ± 0.058 (0.12) | −0.030 ± 0.032 (0.35) | |||
| T/T | 0.072 ± 0.077 (0.35) | −0.119 ± 0.044 (0.008) | |||
| per T allele | 0.014 ± 0.037 (0.70) | −0.053 ± 0.021 (0.01) | −0.026 [−0.074 – 0.023] | ||
| P=0.295 | |||||
| 5848 (rs3213448) | G/G | 0 | 0 | ||
| G/A | −0.046 ± 0.064 (0.47) | −0.041 ± 0.036 (0.25) | |||
| A/A | 0.322 ± 0.200 (0.11) | 0.003 ± 0.117 (0.98) | |||
| per A allele | 0.011 ± 0.055 (0.84) | −0.030 ± 0.031 (0.33) | −0.013 [-0.065 – 0.038] | ||
| P=0.612 | |||||
| 10257 (rs315934) | T/T | 0 | 0 | 0 | |
| T/C | −0.102 ± 0.056 (0.07) | −0.083 ± 0.086 (0.33) | 0.008 ± 0.032 (0.81) | ||
| C/C | −0.022 ± 0.156 (0.88) | −0.270 ± 0.253 (0.29) | −0.087 ± 0.077 (0.26) | ||
| per C allele | −0.072 ± 0.048 (0.14) | −0.098 ± 0.074 (0.19) | −0.012 ± 0.026 (0.64) | −0.033 [−0.078 – 0.012] | |
| P=0.146 | |||||
| 13888 (rs2232354) | T/T | 0 | 0 | 0 | |
| T/G | 0.112 ± 0.055 (0.04) | 0.115 ± 0.070 (0.10) | 0.022 ± 0.032 (0.49) | ||
| G/G | 0.565 ± 0.142 (0.0001) | 0.356 ± 0.163 (0.03) | 0.038 ± 0.072 (0.60) | ||
| per G allele | 0.170 ± 0.050 (0.0001) | 0.141 ± 0.057 (0.013) | 0.021 ± 0.026 (0.47) | 0.103 [−0.002 – 2.08] | |
| P=0.055 | |||||
| 15132 (rs432014) | T/T | 0 | 0 | 0 | |
| T/C | −0.061 ± 0.055 (0.27) | −0.070 ± 0.077 (0.36) | −0.019 ± 0.031 (0.36) | ||
| C/C | 0.086 ± 0.094 (0.36) | 0.037 ± 0.140 (0.79) | −0.154 ± 0.057 (0.79) | ||
| C allele | 0.002 ± 0.040 (0.97) | −0.021 ± 0.057 (0.72) | −0.054 ± 0.023 (0.03) | −0.038 [−0.075 – −0.001] | |
| P=0.056 | |||||
| 15453 (rs380092) | A/A | 0 | 0 | 0 | |
| A/T | −0.090 ± 0.055 (0.10) | −0.105 ± 0.080 (0.19) | −0.008 ± 0.031 (0.81) | ||
| T/T | −0.057 ± 0.095 (0.55) | −0.226 ± 0.138 (0.10) | −0.152 ± 0.052 (0.02) | ||
| per T allele | −0.053 ± 0.040 (0.19) | −0.010 ± 0.059 (0.06) | −0.040 ± 0.023 (0.08) | −0.050 [−0.087 – −0.013] | |
| P=0.008 | |||||
SE = standard error; CI = confidence interval.
Beta coefficients and S.E.’s were estimated using multiple linear regression models, adjusted for age, sex, clinic, BMI, smoking status (and carotid disease status in CLEAR). For each SNP, the first two estimates represent the change in log(CRP) for heterozygote and rare homozygote genotype groups, compared to common homozygotes. The third (and pooled) estimates correspond to the change in (log)CRP associated with each additional copy of the minor allele (assuming an additive genetic model). IL1RN 2765 and 5848 were not typed in the CLEAR subjects.