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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Immunol. 2009 Jun 1;182(11):6744–6752. doi: 10.4049/jimmunol.0804387

Figure 7.

Figure 7

Adoptive cell transfer of Bcl-xL and Survivin transduced CD8+ T cells prevents tumor growth. Naïve CD8+ T cells from OT-I TCR transgenic mice were stimulated with APCs/peptide. On day 2/3, T cells were transduced with retroviral vectors expressing GFP, GFP with Bcl-xL, GFP with Survivin, or GFP with Bcl-xL and Survivin. On day 5 of primary culture, GFP+ CD8+ T cells were sorted and adoptively transferred into naive recipient mice that were subsequently challenged i.p. with EG.7 tumor cells. On day 20, the peritoneal cavity was flushed with 10 ml of ice-cold PBS twice to extract tumor cells and recruited lymphocytes. The total number of cells obtained from each site and the numberof tumor cells from the peritoneal cavity were counted by trypanblue exclusion. Data are mean number of tumor cells ± S.D. from six individual mice (* P<0.05, One-way ANOVA test).