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. Author manuscript; available in PMC: 2010 Aug 4.
Published in final edited form as: Biochemistry. 2009 Aug 4;48(30):7150–7159. doi: 10.1021/bi900457x

Figure 3.

Figure 3

Substrate inhibition of D251A (▲) with ATP, with D251N (◆) as a control. MshC activity was assayed at 2 mM cysteine, 200 μM GlcN-Ins, and varying concentration of ATP at 25 °C as described in the Experimental Procedures section. The line of D251A is a fit of the data to eq 6.