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. Author manuscript; available in PMC: 2009 Nov 28.
Published in final edited form as: J Mol Biol. 2008 Sep 4;383(5):945–956. doi: 10.1016/j.jmb.2008.08.071

Fig. 2.

Fig. 2

PCAF enhances MMP-9 promoter activity and its HAT activity is necessary for optimal induction. (A) Schematic diagram of PCAF and deletion constructs. HAT: histone acetyltransferase activity domain; Bromo: bromodomain. (B) HeLa cells were transiently transfected with the MMP-9-Luc reporter construct and 0 to 0.6 µg of pCX-PCAF, pCX-PCAF ΔHAT1 or pCX-PCAF ΔHAT2, and MMP-9 promoter activity determined as described in Materials and Methods. The results are the mean ± S.E. of at least three independent experiments. * p ≤0.05 compared to pcDNA3 only transfected PMA treated samples; # p ≤0.05 between the two linked samples; NS, not significant compared to pcDNA3 only transfected PMA treated samples. (C) HeLa cells were transiently transfected with 0.4 µg of pcDNA3, pCX-FLAG-PCAF, pCX-FLAG-PCAF ΔHAT1 or pCX-FLAG-PCAF ΔHAT2 for 48 h, and whole cell lysates isolated and subjected to immunoblotting analysis. Actin was utilized as a loading control.