Table 1.
Spectroscopic dataa.
| Residue | Complex | ν,cm−1 | FWHMb, cm−1 |
||
|---|---|---|---|---|---|
| Met1 | Apoenzyme | 2131.4 | (0.4) | 10.9 | (0.5) |
| NADPH | 2132.2 | (0.3) | 10.3 | (0.3) | |
| Folate/NADP+ | 2132.3 | (0.2) | 10.5 | (0.3) | |
| MTX/NADPH | 2132.4 | (0.2) | 10.5 | (0.3) | |
| Folate | 2132.3 | (0.2) | 10.4 | (0.4) | |
| Met16 | Apoenzyme | 2129.7 | (0.4) | 12.3 | (0.2) |
| NADPH | 2132.6 | (0.4) | 9.1 | (0.5) | |
| Folate/NADP+ | 2132.5 | (0.2) | 9.3 | (1.1) | |
| MTX/NADPH | 2133.2 | (0.6) | 9.6 | (1.1) | |
| Folate | 2132.9 | (0.4) | 10.3 | (0.2) | |
| Met20 | Apoenzyme | 2128.6 | (0.4) | 9.8 | (1.0) |
| NADPH | 2132.8 | (0.4) | 9.4 | (1.0) | |
| Folate/NADP+ | 2132.2 | (0.5) | 10.6 | (0.7) | |
| MTX/NADPH | 2130.3 | (1.0) | 12.2 | (0.7) | |
| Folate | 2129.8 | (0.6) | 10.8 | (1.1) | |
| Met42 | Apoenzyme | 2126.2 | (0.2) | 7.3 | (0.3) |
| NADPH | 2125.9 | (0.1) | 7.5 | (0.5) | |
| Folate/NADP+ | 2126.2 | (0.2) | 7.0 | (0.3) | |
| MTX/NADPH | 2125.7 | (0.1) | 7.1 | (0.4) | |
| Folate | 2125.4 | (0.1) | 7.1 | (0.3) | |
a
Standard deviations are in parentheses.
b
Full-width at half maximum.