Skip to main content
. Author manuscript; available in PMC: 2009 Sep 22.
Published in final edited form as: Mol Ther. 2008 Jul 29;16(9):1556–1564. doi: 10.1038/mt.2008.152

Figure 1. Construction of MV-GFP-HAA-IL-13.

Figure 1

(a) Schematic representation of the interleukin-13 (IL-13) receptor α2-retargeted virus. The hemagglutinin (H) protein contains both CD46 and signaling lymphocyte activation molecule–ablating mutations (Y481A and R533A, respectively). IL-13 is displayed on the C-terminus of H protein. The virus also contains the gene encoding green fluorescent protein (GFP) in position 1. (b) Expression levels of viral H protein were determined by western immunoblotting. The retargeted virus expresses chimeric H protein with higher molecular weight (84 kd as compared with 75 kd for the unmodified H protein). Expression levels of IL-13 in viral preparations were determined by western immunoblotting. In contrast to the unmodified virus MV-GFP, the MV-GFP-HAA-IL-13 virus expresses IL-13. (d) Comparable replication of the MV-GFP HAA-IL-13 virus to the unmodified MV-GFP virus was demonstrated in Vero-αHis cells.