Table 2.
Mutations in the TraR N Terminal Domain that are defective in transcriptiona
| Mutation | traI Promoter Activity at the Indicated OOHL Concentrations | |||
|---|---|---|---|---|
| 0.1 nM | 1 nM | 10 nM | 100 nM | |
| Wild type | (100)b | (100)b | (100)b | (100)b |
| Vector Control | <1 | <1 | <1 | <1 |
| D6E | 20 | 15 | 28 | 42 |
| D6G | <1 | <1 | <1 | <1 |
| K7R | 9 | 31 | 28 | 44 |
| D10N | <1 | <1 | <1 | <1 |
| A13L | <1 | 1 | 20 | 7 |
| E15K | 6 | 18 | 31 | 46 |
| D17E | 12 | 11 | 34 | 21 |
| I20W | 5 | 12 | 38 | 14 |
| H44K | 5 | 18 | 34 | 42 |
| P71H | <1 | <1 | <1 | 8 |
| K74E | <1 | 6 | 16 | 8 |
| R75E | 8 | 32 | 41 | 48 |
| R77E | <1 | <1 | 2 | 1 |
| S78E | 12 | 26 | 22 | 28 |
| R79E | 32 | 48 | 36 | 36 |
| K80E | 3 | 19 | 24 | 23 |
| N122A | <1 | 3 | 22 | 29 |
| N122D | <1 | 1 | 10 | 16 |
| G123R | <1 | <1 | <1 | <1 |
| D144R | <1 | <1 | 5 | 23 |
a
All mutations included in this list functioned at levels 50% or lower than wild type TraR in the presence of 100 nM OOHL. Data are presented as a percentage of wild type.
b
Wild type TraR expressed the promoter, a traI-lacZ fusion, at 700, 1500, 2600 and 3000 units of ∃-galactosidase activity at 0.1 nM, 1 nM, 10 nM and 100 nM of OOHL, respectively.