Table 3.

Accumulation in A. tumefaciens and DNA-binding affinity in vitro of all transcription-defective mutants.

Mutation	Accumulation (percent of wild type)	DNA binding (percent of wild type)
Wild type	(100)	(100)
Vector Control	<1	<1
D6E	120	89
D6G	102	70
K7R	78	80
D10N	101	109
A13L	100	65
E15K	45	74
D17E	70	30
I20W	49	57
H44K	27	20
P71H	<10	NTa
K74E	76	74
R75E	88	41
R77E	77	72
S78E	100	103
R79E	68	63
K80E	92	65
N122A	61	100
N122D	33	35
G123R	72	110
D144R	82	74

^aNT, not determined.