Table 4.

Activity of TraR mutants at the TraM promoter.

Mutation	Activity at PtraI 100 nM	Activity at PtraM 100 nM	TraR-dependent PtraM activity
Wild Type	(100)	(100)	86
Vector	<1	14	0
D6E	42	78	64
D6G	<1	16	2
K7R	44	75	61
D10N	<1	15	1
A13L	7	104	90
E15K	46	98	84
D17E	21	70	56
I20W	14	100	86
H44K	42	27	13
K74E	8	36	22
R75E	48	40	26
R77E	1	15	1
S78E	28	37	23
R79E	36	46	32
K80E	23	102	88
N122D	16	33	19
N122A	29	62	48
G123R	<1	35	21
D144R	23	47	33