Table 1.
Study day | Test article administration | Body weight/temperature | Blood sample collectiona | Viremia testing | Immune parametersb | Shedding | CSF examinationc |
---|---|---|---|---|---|---|---|
−2 | X | ||||||
1 | X | X | X | X | X | X | |
5 | X | X | X | X | X | ||
9 | X | X | X | X | X | ||
15 | X | X | X | ||||
22 | X | X | X | ||||
29 | X | X | X | X | |||
57 | X | X | X | X | |||
85 | X | X | X | X | |||
1 year | X | X | X | X | X |
Abbreviations: CEA, carcinoembryonic antigen; CSF, cerebrospinal fluid; MV, measles virus; QRT-PCR, quantitative real-time polymerase chain reaction.
Blood parameters: CEA, hematology, coagulation, and clinical chemistry.
Immune parameters: complement, immunoglobulins, and MV antibody titers.
CSF examination (spinal tap): cell count and differential, morphology review, CEA, total protein, glucose, RNA isolation for QRT-PCR analysis, Vero cell overlay, and anti-measles antibody levels.